LOCUS       pLV-[hsa-miR-601]        9133 bp    DNA     circular     20-APR-2007
SOURCE      
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7054..7914
                     /label=Amp(R)
     promoter        8115..8655
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5659..5893
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6468..6923)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    5965..6095
                     /label=SV40 pA
     misc_feature    6096..6309
                     /label=SV40 ori
     misc_feature    4980..5576
                     /label=WPRE
     promoter        1933..2328
                     /label=human EF1a promoter
     intron          2361..3598
                     /label=intron
     CDS             3645..4970
                     /label=rPuro (RFP Puro)
     misc_feature    3104..3109
                     /label=BamH I / Bgl II
     misc_RNA        3110..3388
                     /label=hsa-mir-601 precursor
BASE COUNT     2175 a      2367 c      2486 g      2105 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgaggatctt agcactgctt 
     3121 ccctttgtcc gcaaaaggag cacagccctc agtctctatc cagacaactc agcggctaca 
     3181 cgctgacttc ctgtgatgcc ttgaaaaagt gcatgagttc gtcttggtct aggattgttg 
     3241 gaggagtcag aaaaactacc ccagggatcc tgaagtcctt tgggtggaaa gggggaggcc 
     3301 cccatgtctg tctggctctc agaaaatggg aaagaaggta agggacatgg caggtcccag 
     3361 tcagagttgg agcaagaagg aaaaaaaagc tagctcgagc ttttggagta cgtcgtcttt 
     3421 aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga 
     3481 agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg 
     3541 atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt 
     3601 gtcgtgaaaa ctacccctct agagtcgagc taccggtcgc caccatggtg agcaagggcg 
     3661 aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct 
     3721 ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca 
     3781 cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc 
     3841 tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg 
     3901 actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg 
     3961 acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca 
     4021 aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca 
     4081 tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga 
     4141 tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct 
     4201 acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca 
     4261 tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc 
     4321 actccaccgg cggcatggac gagctgtaca aggacccacc ggtcgccacc atgaccgagt 
     4381 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg 
     4441 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg 
     4501 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg 
     4561 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag 
     4621 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg 
     4681 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt 
     4741 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg 
     4801 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg 
     4861 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagt 
     4921 gcccgaagga ccgcgcgacc tggtgcatga cccgcaagcc cggtgcctga gcggccgcgt 
     4981 cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt 
     5041 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc 
     5101 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga 
     5161 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc 
     5221 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct 
     5281 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg 
     5341 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct 
     5401 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc 
     5461 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg 
     5521 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat 
     5581 tcgagctcgg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt 
     5641 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt 
     5701 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa 
     5761 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt 
     5821 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg 
     5881 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag 
     5941 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa 
     6001 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt 
     6061 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc 
     6121 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa 
     6181 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt 
     6241 gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta 
     6301 cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca 
     6361 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg 
     6421 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag 
     6481 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag 
     6541 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt 
     6601 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca 
     6661 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata 
     6721 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca 
     6781 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc 
     6841 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa 
     6901 caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct 
     6961 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga 
     7021 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc 
     7081 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg 
     7141 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc 
     7201 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact 
     7261 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc 
     7321 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag 
     7381 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat 
     7441 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt 
     7501 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa 
     7561 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc 
     7621 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg 
     7681 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt 
     7741 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca 
     7801 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat 
     7861 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca 
     7921 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg 
     7981 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg 
     8041 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt 
     8101 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg 
     8161 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata 
     8221 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca 
     8281 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag 
     8341 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc 
     8401 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga 
     8461 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg 
     8521 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac 
     8581 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg 
     8641 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg 
     8701 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct 
     8761 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc 
     8821 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc 
     8881 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg 
     8941 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta 
     9001 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca 
     9061 ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa 
     9121 aagctggagc tgc 
//
